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rpl30 negative  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rpl30 negative
    Rpl30 Negative, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpl30 negative/product/Cell Signaling Technology Inc
    Average 94 stars, based on 5 article reviews
    rpl30 negative - by Bioz Stars, 2026-02
    94/100 stars

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    rpl30 negative  (Cell Signaling Technology Inc)
    94
    Cell Signaling Technology Inc rpl30 negative
    Rpl30 Negative, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpl30 negative/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    rpl30 negative - by Bioz Stars, 2026-02
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    negative control rpl30 primers  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc negative control rpl30 primers
    siAhR suppressed indoxyl sulfate-enhanced vascular inflammation. (A-C) The results of the adhesion assay (A), western blotting detection of adhesion molecules (B) and mRNA expression of E-selectin (C) in HUVEC treated with 0.2 or 2.0 mmol/L indoxyl sulfate for 20 h, followed by tumor necrosis factor-α (TNF-α; 5.7 pmol/L). (D and E) HUVEC were transfected with siAhR or siCont and treated with 10 nmol/L 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) (D) or 100 nmol/L 3-methylcholanthrene (3MC) (E) for 20 h, and then with TNF-α (5.7 pmol/L), followed by real-time quantitative PCR. (F). HUVEC were transfected with siAhR or siCont and treated with indoxyl sulfate for 20 h and then with TNF-α (5.7 pmol/L). mRNA expression of colony-stimulating factor 2 (CSF-2) by real-time quantitative PCR. Data are means ± SD (n = 3). *P < 0.05 vs. siCont and TNF-α (+) and without indoxyl sulfate; **P < 0.01 vs. siCont and TNF-α (+) and without indoxyl sulfate. Data shown are representative of 3 independent experiments. (G) Chromatin was prepared from HUVECs treated with indicated concentration of indoxyl sulfate, followed by stimulation with TNF-α for 30 min and the ChIP assay using anti-AhR antibody. Real-time PCR analysis of the ChIP samples was performed using primers designed to amplify the CSF-2 promoter region and <t>ribosomal</t> <t>protein</t> <t>L30</t> exon3 as negative control. Values are expressed as means means ± SD (n = c3) of a representative of 2 independent experiments. *P < 0.05 vs. TNF-α (+) and without indoxyl sulfate.
    Negative Control Rpl30 Primers, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/negative control rpl30 primers/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    negative control rpl30 primers - by Bioz Stars, 2026-02
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    siAhR suppressed indoxyl sulfate-enhanced vascular inflammation. (A-C) The results of the adhesion assay (A), western blotting detection of adhesion molecules (B) and mRNA expression of E-selectin (C) in HUVEC treated with 0.2 or 2.0 mmol/L indoxyl sulfate for 20 h, followed by tumor necrosis factor-α (TNF-α; 5.7 pmol/L). (D and E) HUVEC were transfected with siAhR or siCont and treated with 10 nmol/L 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) (D) or 100 nmol/L 3-methylcholanthrene (3MC) (E) for 20 h, and then with TNF-α (5.7 pmol/L), followed by real-time quantitative PCR. (F). HUVEC were transfected with siAhR or siCont and treated with indoxyl sulfate for 20 h and then with TNF-α (5.7 pmol/L). mRNA expression of colony-stimulating factor 2 (CSF-2) by real-time quantitative PCR. Data are means ± SD (n = 3). *P < 0.05 vs. siCont and TNF-α (+) and without indoxyl sulfate; **P < 0.01 vs. siCont and TNF-α (+) and without indoxyl sulfate. Data shown are representative of 3 independent experiments. (G) Chromatin was prepared from HUVECs treated with indicated concentration of indoxyl sulfate, followed by stimulation with TNF-α for 30 min and the ChIP assay using anti-AhR antibody. Real-time PCR analysis of the ChIP samples was performed using primers designed to amplify the CSF-2 promoter region and ribosomal protein L30 exon3 as negative control. Values are expressed as means means ± SD (n = c3) of a representative of 2 independent experiments. *P < 0.05 vs. TNF-α (+) and without indoxyl sulfate.

    Journal: Journal of Atherosclerosis and Thrombosis

    Article Title: Crucial Role of the Aryl Hydrocarbon Receptor (AhR) in Indoxyl Sulfate-Induced Vascular Inflammation

    doi: 10.5551/jat.34462

    Figure Lengend Snippet: siAhR suppressed indoxyl sulfate-enhanced vascular inflammation. (A-C) The results of the adhesion assay (A), western blotting detection of adhesion molecules (B) and mRNA expression of E-selectin (C) in HUVEC treated with 0.2 or 2.0 mmol/L indoxyl sulfate for 20 h, followed by tumor necrosis factor-α (TNF-α; 5.7 pmol/L). (D and E) HUVEC were transfected with siAhR or siCont and treated with 10 nmol/L 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) (D) or 100 nmol/L 3-methylcholanthrene (3MC) (E) for 20 h, and then with TNF-α (5.7 pmol/L), followed by real-time quantitative PCR. (F). HUVEC were transfected with siAhR or siCont and treated with indoxyl sulfate for 20 h and then with TNF-α (5.7 pmol/L). mRNA expression of colony-stimulating factor 2 (CSF-2) by real-time quantitative PCR. Data are means ± SD (n = 3). *P < 0.05 vs. siCont and TNF-α (+) and without indoxyl sulfate; **P < 0.01 vs. siCont and TNF-α (+) and without indoxyl sulfate. Data shown are representative of 3 independent experiments. (G) Chromatin was prepared from HUVECs treated with indicated concentration of indoxyl sulfate, followed by stimulation with TNF-α for 30 min and the ChIP assay using anti-AhR antibody. Real-time PCR analysis of the ChIP samples was performed using primers designed to amplify the CSF-2 promoter region and ribosomal protein L30 exon3 as negative control. Values are expressed as means means ± SD (n = c3) of a representative of 2 independent experiments. *P < 0.05 vs. TNF-α (+) and without indoxyl sulfate.

    Article Snippet: Negative control RPL30 primers were obtained from cell signaling technology.

    Techniques: Cell Adhesion Assay, Western Blot, Expressing, Transfection, Real-time Polymerase Chain Reaction, Concentration Assay, Negative Control